Fluorescence Microscopy Imaging Python Packages

ptufile

Read and write PicoQuant PTU and related files (PHU, PCK, PCO, PFS, PUS, PQRES, PQDAT, PQUNI, SPQR, and BIN).

lfdfiles

Laboratory for Fluorescence Dynamics (LFD) file formats.

sdtfile

Read Becker & Hickl SDT files.

phasorpy

An open-source Python library for the analysis of luminescence lifetime and hyperspectral images using the phasor approach.

psf

Point Spread Function calculations for fluorescence microscopy.

liffile

Read Leica image files (LIF, LOF, XLIF, XLCF, XLEF, and LIFEXT).

oiffile

Read Olympus image files (OIF and OIB).

fbdfile

Read FLIMbox data and related files (FBD, FBF, and FBS.XML).

chebyfit

Fit exponential and harmonic functions using Chebyshev polynomials.

efishent

RNA FISH oligos/probes design tool.

tttrlib

File format agnostic low level, high performance API to read and process time-tagged-time resolved (TTTR) data for single-molecule and image spectroscopy)

oirfile

Read Olympus/Evident OIR and POIR files.

ams-bp

Advanced Microscopy Simulations - Create realistic fluorescence microscopy data!

pathml

Tools for computational pathology

icsdll

Interface to the image correlation spectroscopy library ICSx64.dll.

fuse-toolkit

FUSE toolkit supports fluorescent cell image alignment and analysis.

domb-napari

napari plugin for analyzing the redistribution of fluorescence-labeled proteins

pyfrp

PyFRAP: A Python based FRAP analysis tool box

zetastitcher

ZetaStitcher is a tool designed to stitch large volumetric images such as those produced by Light-Sheet Fluorescence Microscopes.

colicoords

Single-cell fluorescence microscopy data analysis

birfi

Blind instrument response function identification from fluorescence decays

obffile

Read Imspector object binary format files (OBF and MSR)

napari-sdtfile

Read Becker & Hickl SDT files.

napari-oiffile

Read Olympus image files (OIF and OIB).